cells

human acute monocytic leukemia

Cell Type:

Monocyte

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

Lymphocyte Growth Medium-3

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	V-001	1e6	67% ±7	40%	Plasmid (general)	0.5 µg	100 µl	I/II/2b
SG	96-FF-100	2e5	65% ±7	81% ±6	Plasmid (general)	0.1 µg	20 µl	96-well
SG	FF-100	2e5	57-63%	53-67%	Plasmid (general)	0.2 µg	20 µl	4D X-Unit
SG	FF-100	1e6	57-59%	46-58%	Plasmid (general)	1 µg	100 µl	4D X-Unit
SG	DV-100	2e5	85%	70%	Plasmid (general)	0.2 µg	20 µl	4D X-Unit

Citations (70)

Generation of locus-specific degradable tag knock-ins in mouse and human cell lines

Categories:

Transfection

Authors:

Damhofer H, Radzisheuskaya A, Helin K.

In:

STAR Protoc (2021) 2(2): 100575

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Maeß MB, Wittig B, Lorkowski S

In:

J Vis Exp (2014) 91: 1-9

Advanced Glycation End Products (AGEs) Cause Pro-atherogenic Changes in Cholesterol Transport: A Possible Mechanism for Cardiovascular Risk in Diabetes

Categories:

Primary Cells and Media

Authors:

Gamez, JD, Voloshyna I, Littlefield MJ, Castro-Magana M, Reiss AB

In:

Other (2014) S11:005: ePub

Advanced Glycation End Products Promote Pro-Atherogenic Changes in Cholesterol Transport: A Possible Mechanism for Cardiovascular Risk in Diabetes

Categories:

Primary Cells and Media

Authors:

Voloshyna I, Gamez-Godoy J, Littlefield MJ, De Leon J, Castro-Magana M, Reiss AB

In:

Other (2014) ePub: ePub

KDT501, a derivative from hops, normalizes glucose metabolism and body weight in rodent models of diabetes

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Konda VR, Desai A, Darland G, Grayson N, Bland JS

In:

PLoS ONE (2014) 9(1): ePub

c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Micheva-Viteva SN, Shou Y, Nowak-Lovato KL, Rector KD, Hong-Geller E

In:

BMC Microbiol. (2013) 13: 249

Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Albright ER1, Kalejta RF

In:

J Virol (2013) 87(17): 9802-12

Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.

Categories:

Primary Cells and Media

Authors:

Qin Q, Penkert RR, Kalejta RF

In:

J Virol (2013) 87(17): 9886-94

Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome.

Categories:

Transfection

Authors:

Busillo JM, Azzam KM, Cidlowski JA.

In:

J Biol Chem (2011) 286(44):: 38703-13

Increased serum and bone matrix levels of transforming growth factor 1 in patients with GH deficiency in response to GH treatment

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay

Authors:

Ueland T, Lekva T, Otterdal K, Dahl TB, Olarescu NC, Jørgensen AP, Fougner KJ, Brixen K, Aukrust P, Bollerslev J

In:

Eur J Endocrinol (2011) 165(3): 393-400

Identification of a Potential Biomarker for FABP4Inhibition: The Power of Lipidomics in Preclinical Drug Testing

Categories:

Serum-free and Speciality Media

Authors:

Karsten Suhre, Werner Römisch-Margl, Martin Hrabé de Angelis,Jerzy Adamski, Gerd Luippold, and Robert Augustin

In:

J Biomol Screen (2011) 16: 5

Overexpression of STARD3 in human monocyte/macrophages induces an anti-atherogenic lipid phenotype.

Categories:

Primary Cells and Media, Transfection

Authors:

Borthwick F, Allen AM, Taylor JM, Graham A

In:

Other (2010) 119(7): 265-72

Efficient non-viral transfection of THP-1 cells

Categories:

Transfection

Authors:

Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S.

In:

J Immunol Methods (2009) 344(2): 109-15

Tribbles-2 is a novel regulator of inflammatory activation of monocytes

Categories:

Transfection

Authors:

Eder K, Guan H, Sung HY, Ward J, Angyal A, Janas M, Sarmay G, Duda E, Turner M, Dower SK, Francis SE, Crossman DC, Kiss-Toth E

In:

Int Immunol (2008) epub: online

Distinct roles of heterogenous nuclear ribonuclear protein K and microrna-16 in cyclooxygenase-2 RNA stability induced by S100B, a ligand of the receptor for advanced glycation end products

Categories:

Transfection

Authors:

Shanmugam N, Reddy MA, Natarajan R

In:

J Biol Chem (2008) epub: online

G9a and HP1 couple histone and DNA methylation to TNFalpha transcription silencing during endotoxin tolerance

Categories:

Transfection

Authors:

El Gazzar M, Yoza BK, Chen X, Hu J, Hawkins G, McCall CE

In:

J Biol Chem (2008) epub: online

Beta-PIX and Rac1 GTPase mediate trafficking and negative regulation of NOD2

Categories:

Transfection

Authors:

Eitel J, Kruell M, Hocke AC, N'Guessan PD, Zahlten J, Schmeck B, Slevogt H, Hippenstiel S, Suttorp N, Opitz B

In:

J Immunol (2008) 181(4): 2664-71

Cathepsin B is involved in the trafficking of TNF-alpha-containing vesicles to the plasma membrane in macrophages

Categories:

Transfection

Authors:

Ha SD, Martins A, Khazaie K, Han J, Chan BM, Kim SO

In:

J Immunol (2008) 181(1): 690-7

Activation of the Adenosine-A3 Receptor Stimulates Matrix Metalloproteinase-9 Secretion by Macrophages

Categories:

Transfection

Authors:

Velot E, Haas B, Léonard F, Ernens I, Rolland-Turner M, Schwartz C, Longrois D, Devaux Y, Wagner DR

In:

Cardiovasc Res (2008) epub: online

Upregulation of the TLR3 pathway by Kaposi's sarcoma-associated herpesvirus during primary infection

Categories:

Transfection

Authors:

West J, Damania B

In:

J Virol (2008) 82(11): 5440-9

How cells tiptoe on adhesive surfaces before sticking

Categories:

Transfection

Authors:

Pierres A, Benoliel AM, Touchard D, Bongrand P

In:

Biophys J (2008) 94(10): 4114-22

NAIP and Ipaf control Legionella pneumophila replication in human cells

Categories:

Transfection

Authors:

Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B

In:

J Immunol (2008) 180(10): 6808-15

Nitric oxide-p38 MAPK signaling stabilizes mRNA through AU-rich element-dependent and -independent mechanisms

Categories:

Transfection

Authors:

Wang S, Zhang J, Zhang Y, Kern S, Danner RL

In:

J Leukoc Biol (2008) 83(4): 982-90

Wiskott-aldrich syndrome protein is a key regulator of the phagocytic cup formation in macrophages

Categories:

Transfection

Authors:

Tsuboi S, Meerloo J

In:

J Biol Chem (2007) 282(47): 34194-203

Membrane translocation of P-Rex1 is mediated by G protein beta gamma subunits and phosphoinositide 3-kinase

Categories:

Transfection

Authors:

Barber MA, Donald S, Thelen S, Anderson KE, Thelen M, Welch HC

In:

J Biol Chem (2007) 282(41): 29967-76

Microbial Products Stimulate Human Toll-like Receptor 2 Expression through Histone Modification Surrounding a Proximal NF-B-binding Site

Categories:

Transfection

Authors:

Johnson CM, Tapping RI

In:

J Biol Chem (2007) 282(43): 31197-205

Human cytomegalovirus gene expression is silenced by the Daxx-mediated intrinsic immune defense when model latent infections are established in vitro

Categories:

Transfection

Authors:

Saffert RT, Kalejta RF

In:

J Virol (2007) 81(17): 9109-20

Epigenetic silencing of TNFalpha during endotoxin tolerance

Categories:

Transfection

Authors:

El Gazzar M, Yoza BK, Hu JY, Cousart SL, McCall CE

In:

J Biol Chem (2007) 282(37): 26857-64

The molecular mechanisms underlying the proinflammatory actions of thiazolidinediones in human macrophages

Categories:

Transfection

Authors:

Hall JM, McDonnell DP

In:

Mol Endocrinol (2007) 21(8): 1756-68

The localization and activity of sphingosine kinase 1 are coordinately-regulated with actin cytoskeletal dynamics in macrophages

Categories:

Transfection

Authors:

Kusner DJ, Thompson CR, Melrose NA, Pitson SM, Obeid LM, Iyer SS

In:

J Biol Chem (2007) 282(32): 23147-62

Modulation of the Cellular Accumulation and Intracellular Activity of Daptomycin towards phagocytized Staphylococcus aureus by the P-glycoprotein (MDR1) Efflux Transporter in human THP-1 macrophages and Madin-Darby canine kidney cells

Categories:

Transfection

Authors:

Lemaire S, Van Bambeke F, Mingeot-Leclercq MP, Tulkens PM

In:

Antimicrob Agents Chemother (2007) 51(8): 2748-57

Binding of caspase-3 prodomain to heat shock protein 27 regulates monocyte apoptosis by inhibiting caspase-3 proteolytic activation

Categories:

Transfection

Authors:

Voss OH, Batra S, Kolattukudy SJ, Gonzalez-Mejia ME, Smith JB, Doseff AI

In:

J Biol Chem (2007) 282(34): 25088-99

Cutting Edge: Vitamin D-Mediated Human Antimicrobial Activity against Mycobacterium tuberculosis Is Dependent on the Induction of Cathelicidin

Categories:

Transfection

Authors:

Liu PT, Stenger S, Tang DH, Modlin RL

In:

J Immunol (2007) 179(4): 2060-3

ATP-binding cassette transporter A1 gene transcription is downregulated by activator protein 2alpha. Doxazosin inhibits activator protein 2alpha and increases high-density lipoprotein biogenesis independent of alpha1-adrenoceptor blockade

Categories:

Transfection

Authors:

Iwamoto N, Abe-Dohmae S, Ayaori M, Tanaka N, Kusuhara M, Ohsuzu F, Yokoyama S

In:

Circ Res (2007) 101(2): 156-65

Phosphorylation of pleckstrin increases proinflammatory cytokine secretion by mononuclear phagocytes in diabetes mellitus

Categories:

Transfection

Authors:

Ding Y, Kantarci A, Badwey JA, Hasturk H, Malabanan A, Van Dyke TE

In:

J Immunol (2007) 179(1): 647-54

Epigenetic regulation of tumor necrosis factor alpha

Categories:

Transfection

Authors:

Sullivan KE, Reddy AB, Dietzmann K, Suriano AR, Kocieda VP, Stewart M, Bhatia M

In:

Mol Cell Biol (2007) 27(14): 5147-60

Pyrin Levels in Human Monocytes and Monocyte-Derived Macrophages Regulate IL-1beta Processing and Release

Categories:

Transfection

Authors:

Seshadri S, Duncan MD, Hart JM, Gavrilin MA, Wewers MD

In:

J Immunol (2007) 179(2): 1274-81

Differential regulation and role of interleukin-1 receptor associated kinase-M in innate immunity signaling

Categories:

Transfection

Authors:

Su J, Xie Q, Wilson I, Li L

In:

Cell Signal (2007) 19(7): 1596-601

Cryopyrin/NALP3 binds ATP/dATP, is an ATPase, and requires ATP binding to mediate inflammatory signaling

Categories:

Transfection

Authors:

Duncan JA, Bergstralh DT, Wang Y, Willingham SB, Ye Z, Zimmermann AG, Ting JP

In:

Proc Natl Acad Sci USA (2007) 104(19): 8041-6

Disease-associated mutations in CIAS1 induce cathepsin B-dependent rapid cell death of human THP-1 monocytic cells

Categories:

Transfection

Authors:

Fujisawa A, Kambe N, Saito M, Nishikomori R, Tanizaki H, Kanazawa N, Adachi S, Heike T, Sagara J, Suda T, Nakahata T, Miyachi Y

In:

Blood (2007) 109(7): 2903-11

Phospholipid Transfer Protein Augments Apoptosis in THP-1-Derived Macrophages Induced by Lipolyzed Hypertriglyceridemic Plasma

Categories:

Transfection

Authors:

Wehinger A, Tancevski I, Schgoer W, Eller P, Hochegger K, Morak M, Hermetter A, Ritsch A, Patsch JR, Foeger B

In:

Arterioscler Thromb Vasc Biol (2007) 27(4): 908-15

Identification of molecular predictors of response in a study of tipifarnib treatment in relapsed and refractory acute myelogenous leukemia

Categories:

Transfection

Authors:

Raponi M, Harousseau JL, Lancet JE, Lowenberg B, Stone R, Zhang Y, Rackoff W, Wang Y, Atkins D

In:

Clin Cancer Res (2007) 13(7): 2254-60

Retinoic Acid regulates CD1d gene expression at the transcriptional level in human and rodent monocytic cells

Categories:

Transfection

Authors:

Chen Q, Ross AC

In:

Exp Biol Med (2007) 232(4): 488-94

Tyrosine kinase Syk associates with toll-like receptor 4 and regulates signaling in human monocytic cells.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Chaudhary A, Fresquez TM, Naranjo MJ.

In:

Immunol Cell Biol (2007) 85(3): 249-56

Requirement for a Complex of Wiskott-Aldrich Syndrome Protein (WASP) with WASP Interacting Protein in Podosome Formation in Macrophages

Categories:

Transfection

Authors:

Tsuboi S

In:

J Immunol (2007) 178(5): 2987-95

Stable Over-Expression of Human Macrophage Cholesteryl Ester Hydrolase (CEH) Results in Enhanced Free Cholesterol Efflux from Human THP1-macrophages

Categories:

Transfection

Authors:

Zhao B, Song J, St Clair R, Ghosh S

In:

Am J Physiol Cell Physiol (2007) 292(1): C405-12

Monocyte differentiation up-regulates the expression of the lysosomal sialidase, Neu1, and triggers its targeting to the plasma membrane via major histocompatibility complex class II-positive compartments

Categories:

Transfection

Authors:

Liang F, Seyrantepe V, Landry K, Ahmad R, Ahmad A, Stamatos NM, Pshezhetsky AV

In:

J Biol Chem (2006) 281(37): 27526-38

Induction of RelB Participates in Endotoxin Tolerance

Categories:

Transfection

Authors:

Yoza BK, Hu JY, Cousart SL, Forrest LM, McCall CE

In:

J Immunol (2006) 177(6): 4080-5

The HTLV-I p30 Interferes with TLR4 signaling and modulates the release of pro-and anti-inflammatory cytokines from human macrophages

Categories:

Transfection

Authors:

Datta A, Sinha-Datta U, Kaur Dhillon N, Buch S, Nicot C

In:

J Biol Chem (2006) 281(33): 23414-24

Activation of hypoxia-inducible factor 1 during macrophage differentiation

Categories:

Transfection

Authors:

Oda T, Hirota K, Nishi K, Takabuchi S, Oda S, Yamada H, Arai T, Fukuda K, Kita T, Adachi T, Semenza GL, Nohara R

In:

Am J Physiol Cell Physiol (2006) 291(1): C104-13

G1 cell-cycle arrest and apoptosis by histone deacetylase inhibition in MLL-AF9 acute myeloid leukemia cells is p21 dependent and MLL-AF9 independent

Categories:

Transfection

Authors:

Tonelli R, Sartini R, Fronza R, Freccero F, Franzoni M, Dongiovanni D, Ballarini M, Ferrari S, D'apolito M, Di Cola G, Capranico G, Khobta A, Campanini R, Paolucci P, Minucci S, Pession A

In:

Leukemia (2006) 20(7): 1307-10

Nitric oxide activation of Erk1/2 regulates the stability and translation of mRNA transcripts containing CU-rich elements

Categories:

Transfection

Authors:

Wang S, Zhang J, Theel S, Barb JJ, Munson PJ, Danner RL

In:

Nucleic Acids Res (2006) 34(10): 3044-56

Atherosclerotic Plaque Macrophage Transcriptional Regulators Are Expressed in Blood and Modulated by Tristetraprolin

Categories:

Transfection

Authors:

Patino WD, Kang JG, Matoba S, Mian OY, Gochuico BR, Hwang PM

In:

Circ Res (2006) 98(10): 1282-9

Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation

Categories:

Transfection

Authors:

Lai JP, Ho WZ, Kilpatrick LE, Wang X, Tuluc F, Korchak HM, Douglas SD

In:

Proc Natl Acad Sci USA (2006) 103(20): 7771-7776

ASC Directs NF-B Activation by Regulating Receptor Interacting Protein-2 (RIP2) Caspase-1 Interactions

Categories:

Transfection

Authors:

Sarkar A, Duncan M, Hart J, Hertlein E, Guttridge DC, Wewers MD

In:

J Immunol (2006) 176(8): 4979-86

CpG-induced tyrosine phosphorylation occurs via a TLR9-independent mechanism and is required for cytokine secretion

Categories:

Transfection

Authors:

Sanjuan MA, Rao N, Lai KT, Gu Y, Sun S, Fuchs A, Fung-Leung WP, Colonna M, Karlsson L

In:

J Cell Biol (2006) 172(7): 1057-68

Association of beta-arrestin and TRAF6 negatively regulates Toll-like receptor-interleukin 1 receptor signaling

Categories:

Transfection

Authors:

Wang Y, Tang Y, Teng L, Wu Y, Zhao X and Pei G

In:

Nat Immunol (2006) 7(2): 139-47

Functional characterization of human cysteinyl leukotriene 1 receptor gene structure

Categories:

Transfection

Authors:

Woszczek G, Pawliczak R, Qi HY, Nagineni S, Alsaaty S, Logun C and Shelhamer JH

In:

J Immunol (2005) 175(8): 5152-5159

PU.1 Regulates the Tissue-specific Expression of Dendritic Cell-specific Intercellular Adhesion Molecule (ICAM)-3-grabbing Nonintegrin

Categories:

Transfection

Authors:

Dominguez-Soto A, Puig-Kroger A, Vega MA and Corbi AL

In:

J Biol Chem (2005) 280(39): 33123-33131

HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis

Categories:

Transfection

Authors:

Drakesmith H, Chen N, Ledermann H, Screaton G, Townsend A and Xu XN

In:

Proc Natl Acad Sci USA (2005) 102(31): 11017-11022

Tyrosine phosphorylation regulates maturation of receptor tyrosine kinases

Categories:

Transfection

Authors:

Schmidt-Arras DE, Bohmer A, Markova B, Choudhary C, Serve H and Bohmer FD

In:

Mol Cell Biol (2005) 25(9): 3690-3703

The interferon regulatory factor, IRF5, is a central mediator of TLR7 signaling

Categories:

Transfection

Authors:

Schoenemeyer A, Barnes BJ, Mancl ME, Latz E, Goutagny N, Pitha PM, Fitzgerald KA and Golenbock DT

In:

J Biol Chem (2005) 280(17): 17005-17012

Regulation of monocyte apoptosis by the protein kinase C delta (PKCdelta )-dependent phosphorylation of caspase-3

Categories:

Transfection

Authors:

Voss OH, Kim S, Wewers MD and Doseff AI

In:

J Biol Chem (2005) 280(17): 17371-17379

Circulating transcriptome reveals markers of atherosclerosis

Categories:

Transfection

Authors:

Patino WD, Mian OY, Kang JG, Matoba S, Bartlett LD, Holbrook B, Trout HH 3rd, Kozloff L and Hwang PM

In:

Proc Natl Acad Sci USA (2005) 102(9): 3423-3428

Early steps of clathrin-mediated endocytosis involved in phagosomal escape of Fcgamma receptor-targeted adenovirus

Categories:

Transfection

Authors:

Meier O, Gastaldelli M, Boucke K, Hemmi S and Greber UF

In:

J Virol (2005) 79(4): 2604-2613

SHIP2 Is Recruited to the Cell Membrane upon Macrophage Colony-Stimulating Factor (M-CSF) Stimulation and Regulates M-CSF-Induced Signaling

Categories:

Transfection

Authors:

Wang Y, Keogh RJ, Hunter MG, Mitchell CA, Frey RS, Javaid K, Malik AB, Schurmans S, Tridandapani S, Marsh CB

In:

J Immunol (2004) 173(11): 6820-6830

Translocation of the inhibitor of apoptosis protein c-IAP1 from the nucleus to the Golgi in hematopoietic cells undergoing differentiation: a nuclear export signal-mediated event

Categories:

Transfection

Authors:

Plenchette S, Cathelin S, Rebe C, Launay S, Ladoire S, Sordet O, Ponnelle T, Debili N, Phan TH, Padua RA, Dubrez-Daloz L and Solary E

In:

Blood (2004) 104(7): 2035-2043

Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways

Categories:

Transfection

Authors:

Gao H, Sun Y, Wu Y, Luan B, Wang Y, Qu B and Pei G

In:

Mol Cell (2004) 14(3): 303-317

Amyloid precursor protein mediates proinflammatory activation of monocytic lineage cells

Categories:

Transfection

Authors:

Sondag CM and Combs CK

In:

J Biol Chem (2004) 279(14): 14456-14463

Nucleofection as an efficient nonviral transfection method for human monocytic cells

Categories:

Transfection

Authors:

Martinet W, Schrijvers DM and Kockx MM

In:

Biotechnol Lett (2003) 25: 1025-1029

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