THP-1

human acute monocytic leukemia
Cell Type:
Monocyte
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V V-001 1e6 67% ±7 40% Plasmid (general) 0.5 µg 100 µl I/II/2b
SG 96-FF-100 2e5 65% ±7 81% ±6 Plasmid (general) 0.1 µg 20 µl Shuttle
SG

FF-100

2e5 57-63% 53-67% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
SG

FF-100

1e6 57-59% 46-58% Plasmid (general) 1 µg 100 µl 4D X-Unit

Citations (68)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Maeß MB, Wittig B, Lorkowski S 
In:
J Vis Exp (2014) 91: 1-9 
Categories:
Primary Cells and Media 
Authors:
Gamez, JD, Voloshyna I, Littlefield MJ, Castro-Magana M, Reiss AB 
In:
Other (2014) S11:005: ePub 
Categories:
Primary Cells and Media 
Authors:
Voloshyna I, Gamez-Godoy J, Littlefield MJ, De Leon J, Castro-Magana M, Reiss AB 
In:
Other (2014) ePub: ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Konda VR, Desai A, Darland G, Grayson N, Bland JS 
In:
PLoS ONE (2014) 9(1): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Micheva-Viteva SN, Shou Y, Nowak-Lovato KL, Rector KD, Hong-Geller E 
In:
BMC Microbiol. (2013) 13: 249 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Categories:
Primary Cells and Media 
Authors:
Qin Q, Penkert RR, Kalejta RF 
In:
J Virol (2013) 87(17): 9886-94 
Categories:
Transfection 
Authors:
Busillo JM, Azzam KM, Cidlowski JA. 
In:
J Biol Chem (2011) 286(44):: 38703-13 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay  
Authors:
Ueland T, Lekva T, Otterdal K, Dahl TB, Olarescu NC, Jørgensen AP, Fougner KJ, Brixen K, Aukrust P, Bollerslev J 
In:
Eur J Endocrinol (2011) 165(3): 393-400 
Categories:
Primary Cells and Media, Transfection 
Authors:
Borthwick F, Allen AM, Taylor JM, Graham A 
In:
Other (2010) 119(7): 265-72 
Categories:
Transfection 
Authors:
Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S. 
In:
J Immunol Methods (2009) 344(2): 109-15 
Categories:
Transfection 
Authors:
Eder K, Guan H, Sung HY, Ward J, Angyal A, Janas M, Sarmay G, Duda E, Turner M, Dower SK, Francis SE, Crossman DC, Kiss-Toth E 
In:
Int Immunol (2008) epub: online 
Categories:
Transfection 
Authors:
El Gazzar M, Yoza BK, Chen X, Hu J, Hawkins G, McCall CE 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Eitel J, Kruell M, Hocke AC, N'Guessan PD, Zahlten J, Schmeck B, Slevogt H, Hippenstiel S, Suttorp N, Opitz B 
In:
J Immunol (2008) 181(4): 2664-71 
Categories:
Transfection 
Authors:
Ha SD, Martins A, Khazaie K, Han J, Chan BM, Kim SO 
In:
J Immunol (2008) 181(1): 690-7 
Categories:
Transfection 
Authors:
Velot E, Haas B, Léonard F, Ernens I, Rolland-Turner M, Schwartz C, Longrois D, Devaux Y, Wagner DR 
In:
Cardiovasc Res (2008) epub: online 
Categories:
Transfection 
Authors:
West J, Damania B 
In:
J Virol (2008) 82(11): 5440-9 
Categories:
Transfection 
Authors:
Pierres A, Benoliel AM, Touchard D, Bongrand P 
In:
Biophys J (2008) 94(10): 4114-22 
Categories:
Transfection 
Authors:
Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B 
In:
J Immunol (2008) 180(10): 6808-15 
Categories:
Transfection 
Authors:
Wang S, Zhang J, Zhang Y, Kern S, Danner RL 
In:
J Leukoc Biol (2008) 83(4): 982-90 
Categories:
Transfection 
Authors:
Tsuboi S, Meerloo J 
In:
J Biol Chem (2007) 282(47): 34194-203 
Categories:
Transfection 
Authors:
Barber MA, Donald S, Thelen S, Anderson KE, Thelen M, Welch HC 
In:
J Biol Chem (2007) 282(41): 29967-76 
Categories:
Transfection 
Authors:
Johnson CM, Tapping RI 
In:
J Biol Chem (2007) 282(43): 31197-205 
Categories:
Transfection 
Authors:
El Gazzar M, Yoza BK, Hu JY, Cousart SL, McCall CE 
In:
J Biol Chem (2007) 282(37): 26857-64 
Categories:
Transfection 
Authors:
Hall JM, McDonnell DP 
In:
Mol Endocrinol (2007) 21(8): 1756-68 
Categories:
Transfection 
Authors:
Kusner DJ, Thompson CR, Melrose NA, Pitson SM, Obeid LM, Iyer SS 
In:
J Biol Chem (2007) 282(32): 23147-62 
Categories:
Transfection 
Authors:
Voss OH, Batra S, Kolattukudy SJ, Gonzalez-Mejia ME, Smith JB, Doseff AI 
In:
J Biol Chem (2007) 282(34): 25088-99 
Categories:
Transfection 
Authors:
Liu PT, Stenger S, Tang DH, Modlin RL 
In:
J Immunol (2007) 179(4): 2060-3 
Categories:
Transfection 
Authors:
Ding Y, Kantarci A, Badwey JA, Hasturk H, Malabanan A, Van Dyke TE 
In:
J Immunol (2007) 179(1): 647-54 
Categories:
Transfection 
Authors:
Sullivan KE, Reddy AB, Dietzmann K, Suriano AR, Kocieda VP, Stewart M, Bhatia M 
In:
Mol Cell Biol (2007) 27(14): 5147-60 
Categories:
Transfection 
Authors:
Seshadri S, Duncan MD, Hart JM, Gavrilin MA, Wewers MD 
In:
J Immunol (2007) 179(2): 1274-81 
Categories:
Transfection 
Authors:
Su J, Xie Q, Wilson I, Li L 
In:
Cell Signal (2007) 19(7): 1596-601 
Categories:
Transfection 
Authors:
Duncan JA, Bergstralh DT, Wang Y, Willingham SB, Ye Z, Zimmermann AG, Ting JP 
In:
Proc Natl Acad Sci USA (2007) 104(19): 8041-6 
Categories:
Transfection 
Authors:
Fujisawa A, Kambe N, Saito M, Nishikomori R, Tanizaki H, Kanazawa N, Adachi S, Heike T, Sagara J, Suda T, Nakahata T, Miyachi Y 
In:
Blood (2007) 109(7): 2903-11 
Categories:
Transfection 
Authors:
Wehinger A, Tancevski I, Schgoer W, Eller P, Hochegger K, Morak M, Hermetter A, Ritsch A, Patsch JR, Foeger B 
In:
Arterioscler Thromb Vasc Biol (2007) 27(4): 908-15 
Categories:
Transfection 
Authors:
Raponi M, Harousseau JL, Lancet JE, Lowenberg B, Stone R, Zhang Y, Rackoff W, Wang Y, Atkins D 
In:
Clin Cancer Res (2007) 13(7): 2254-60 
Categories:
Transfection 
Authors:
Chen Q, Ross AC 
In:
Exp Biol Med (2007) 232(4): 488-94 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chaudhary A, Fresquez TM, Naranjo MJ. 
In:
Immunol Cell Biol (2007) 85(3): 249-56 
Categories:
Transfection 
Authors:
Zhao B, Song J, St Clair R, Ghosh S 
In:
Am J Physiol Cell Physiol (2007) 292(1): C405-12 
Categories:
Transfection 
Authors:
Liang F, Seyrantepe V, Landry K, Ahmad R, Ahmad A, Stamatos NM, Pshezhetsky AV 
In:
J Biol Chem (2006) 281(37): 27526-38 
Categories:
Transfection 
Authors:
Yoza BK, Hu JY, Cousart SL, Forrest LM, McCall CE 
In:
J Immunol (2006) 177(6): 4080-5 
Categories:
Transfection 
Authors:
Datta A, Sinha-Datta U, Kaur Dhillon N, Buch S, Nicot C 
In:
J Biol Chem (2006) 281(33): 23414-24 
Categories:
Transfection 
Authors:
Oda T, Hirota K, Nishi K, Takabuchi S, Oda S, Yamada H, Arai T, Fukuda K, Kita T, Adachi T, Semenza GL, Nohara R 
In:
Am J Physiol Cell Physiol (2006) 291(1): C104-13 
Categories:
Transfection 
Authors:
Tonelli R, Sartini R, Fronza R, Freccero F, Franzoni M, Dongiovanni D, Ballarini M, Ferrari S, D'apolito M, Di Cola G, Capranico G, Khobta A, Campanini R, Paolucci P, Minucci S, Pession A 
In:
Leukemia (2006) 20(7): 1307-10 
Categories:
Transfection 
Authors:
Wang S, Zhang J, Theel S, Barb JJ, Munson PJ, Danner RL 
In:
Nucleic Acids Res (2006) 34(10): 3044-56 
Categories:
Transfection 
Authors:
Patino WD, Kang JG, Matoba S, Mian OY, Gochuico BR, Hwang PM 
In:
Circ Res (2006) 98(10): 1282-9 
Categories:
Transfection 
Authors:
Lai JP, Ho WZ, Kilpatrick LE, Wang X, Tuluc F, Korchak HM, Douglas SD 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7771-7776 
Categories:
Transfection 
Authors:
Sarkar A, Duncan M, Hart J, Hertlein E, Guttridge DC, Wewers MD 
In:
J Immunol (2006) 176(8): 4979-86 
Categories:
Transfection 
Authors:
Sanjuan MA, Rao N, Lai KT, Gu Y, Sun S, Fuchs A, Fung-Leung WP, Colonna M, Karlsson L 
In:
J Cell Biol (2006) 172(7): 1057-68 
Categories:
Transfection 
Authors:
Wang Y, Tang Y, Teng L, Wu Y, Zhao X and Pei G 
In:
Nat Immunol (2006) 7(2): 139-47 
Categories:
Transfection 
Authors:
Woszczek G, Pawliczak R, Qi HY, Nagineni S, Alsaaty S, Logun C and Shelhamer JH 
In:
J Immunol (2005) 175(8): 5152-5159 
Categories:
Transfection 
Authors:
Dominguez-Soto A, Puig-Kroger A, Vega MA and Corbi AL 
In:
J Biol Chem (2005) 280(39): 33123-33131 
Categories:
Transfection 
Authors:
Drakesmith H, Chen N, Ledermann H, Screaton G, Townsend A and Xu XN 
In:
Proc Natl Acad Sci USA (2005) 102(31): 11017-11022 
Categories:
Transfection 
Authors:
Schmidt-Arras DE, Bohmer A, Markova B, Choudhary C, Serve H and Bohmer FD 
In:
Mol Cell Biol (2005) 25(9): 3690-3703 
Categories:
Transfection 
Authors:
Schoenemeyer A, Barnes BJ, Mancl ME, Latz E, Goutagny N, Pitha PM, Fitzgerald KA and Golenbock DT 
In:
J Biol Chem (2005) 280(17): 17005-17012 
Categories:
Transfection 
Authors:
Voss OH, Kim S, Wewers MD and Doseff AI 
In:
J Biol Chem (2005) 280(17): 17371-17379 
Categories:
Transfection 
Authors:
Patino WD, Mian OY, Kang JG, Matoba S, Bartlett LD, Holbrook B, Trout HH 3rd, Kozloff L and Hwang PM 
In:
Proc Natl Acad Sci USA (2005) 102(9): 3423-3428 
Categories:
Transfection 
Authors:
Meier O, Gastaldelli M, Boucke K, Hemmi S and Greber UF 
In:
J Virol (2005) 79(4): 2604-2613 
Categories:
Transfection 
Authors:
Wang Y, Keogh RJ, Hunter MG, Mitchell CA, Frey RS, Javaid K, Malik AB, Schurmans S, Tridandapani S, Marsh CB 
In:
J Immunol (2004) 173(11): 6820-6830 
Categories:
Transfection 
Authors:
Plenchette S, Cathelin S, Rebe C, Launay S, Ladoire S, Sordet O, Ponnelle T, Debili N, Phan TH, Padua RA, Dubrez-Daloz L and Solary E 
In:
Blood (2004) 104(7): 2035-2043 
Categories:
Transfection 
Authors:
Gao H, Sun Y, Wu Y, Luan B, Wang Y, Qu B and Pei G 
In:
Mol Cell (2004) 14(3): 303-317 
Categories:
Transfection 
Authors:
Sondag CM and Combs CK 
In:
J Biol Chem (2004) 279(14): 14456-14463 
Categories:
Transfection 
Authors:
Martinet W, Schrijvers DM and Kockx MM 
In:
Biotechnol Lett (2003) 25: 1025-1029 
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