cells

mouse thymoma, CD4+, CD8+, expresses the AND TCR (PMID: 2571940), recognizes pigeon cytochrome c peptide on the I-Ek class II MHC molecule. When plated on a layer of APCs expressing I-Ek and ICAM (DCEK-ICAM cells), DPK cells differentiate from DP CD4+ CD8

Cell Type:

T Cell

Tissue Origin:

thymus

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	B-013	8e6			siRNA	225	100 µl	I/II/2b

Citations

Histone Deacetylase 7 Functions as a Key Regulator of Genes Involved in Both Positive and Negative Selection of Thymocytes

Categories:

Transfection

Authors:

Kasler HG, Verdin E

In:

Mol Cell Biol (2007) 27(14): 5184-200