PC-12

rat adrenal pheochromocytoma

Cell Type:
Adrenal Cells
Tissue Origin:
adrenal
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EI-100 2e6 82% ±1 54% ±6 Plasmid (general) 2 µg 100 µl 4D X-Unit
SF EI-100 4e5 83% ±2 57% ±4 Plasmid (general) 400 ng 20 µl 4D X-Unit
V U-029 2e6 92.3% ±3 80.8% ±5 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (25)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
De Nadai T, Marchetti L, Di Rienzo C, Calvello M, Signore G3, Di Matteo P, Gobbo F, Turturro S, Meucci S, Viegi A, Beltram F, Luin S, Cattaneo A 
In:
Scientific Reports (2016) 1(6): ePub 
Categories:
Transfection 
Authors:
Ferron L, Davies A, Page KM, Cox DJ, Leroy J, Waithe D, Butcher AJ, Sellaturay P, Bolsover S, Pratt WS, Moss FJ, Dolphin AC 
In:
J Neurosci (2008) 28(42): 10604-17 
Categories:
Transfection 
Authors:
Ravni A, Vaudry D, Gerdin MJ, Eiden MV, Falluel-Morel A, Gonzalez B, Vaudry H, Eiden LE 
In:
Mol Pharmacol (2008) 73(6): 1688-708 
Categories:
Transfection 
Authors:
Rogemond V, Auger C, Giraudon P, Becchi M, Auvergnon N, Belin MF, Honnorat J, Moradi-Améli M 
In:
J Biol Chem (2008) 283(21): 14751-61 
Categories:
Transfection 
Authors:
Hausott B, Schlick B, Vallant N, Dorn R, Klimaschewski L 
In:
Neuroscience (2008) 153(2): 461-73 
Categories:
Transfection 
Authors:
Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ 
In:
J Neurosci (2008) 28(11): 2753-65 
Categories:
Transfection 
Authors:
Suzuki F, Morishima S, Tanaka T, Muramatsu I 
In:
J Biol Chem (2007) 282(40): 29563-73 
Categories:
Transfection 
Authors:
Mishra R, Barthwal MK, Sondarva G, Rana B, Wong L, Chatterjee M, Woodgett JR, Rana A 
In:
J Biol Chem (2007) 282(42): 30393-405 
Categories:
Transfection 
Authors:
Gehlhaus M, Schmitt N, Volk B, Meyer R 
In:
J Pharmacol Exp Ther (2007) 322(2): 550-9 
Categories:
Transfection 
Authors:
Chivatakarn O, Kaneko S, He Z, Tessier-Lavigne M, Giger RJ 
In:
J Neurosci (2007) 27(27): 7117-24 
Categories:
Transfection 
Authors:
van Niekerk EA, Willis DE, Chang JH, Reumann K, Heise T, Twiss JL 
In:
Proc Natl Acad Sci USA (2007) 104(31): 12913-8 
Categories:
Transfection 
Authors:
Conrad S, Genth H, Hofmann F, Just I, Skutella T 
In:
J Biol Chem (2007) 282(22): 16423-33 
Categories:
Transfection 
Authors:
Seng S, Avraham HK, Jiang S, Venkatesh S, Avraham S 
In:
Mol Cell Biol (2006) 26(22): 8371-84 
Categories:
Transfection 
Authors:
von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS 
In:
Nat Cell Biol (2006) 8(9): 1011-6 
Categories:
Transfection 
Authors:
Malacombe M, Ceridono M, Calco V, Chasserot-Golaz S, McPherson PS, Bader MF, Gasman S 
In:
EMBO J (2006) 25(15): 3494-503 
Categories:
Transfection 
Authors:
White C, Yang J, Monteiro MJ, Foskett JK 
In:
J Biol Chem (2006) 281(30): 20825-20833 
Categories:
Transfection 
Authors:
Meyer MZ, Deliot N, Chasserot-Golaz S, Premont RT, Bader MF, Vitale N 
In:
J Biol Chem (2006) 281(12): 7919-26 
Categories:
Transfection 
Authors:
Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K 
In:
Mol Biol Cell (2006) 17(1): 122-129 
Categories:
Transfection 
Authors:
Garcia AL, Han SK, Janssen WG, Khaing ZZ, Ito T, Glucksman MJ, Benson DL and Salton SR 
In:
J Biol Chem (2005) 280(50): 41595-41608 
Categories:
Transfection 
Authors:
Tyurina YY, Nylander KD, Mirnics ZK, Portugal C, Yan C, Zaccaro C, Saragovi HU, Kagan VE and Schor NF 
In:
Aging Cell (2005) 4(4): 187-196 
Categories:
Transfection 
Authors:
Yoshizawa M, Kawauchi T, Sone M, Nishimura YV, Terao M, Chihama K, Nabeshima Y and Hoshino M 
In:
J Neurosci (2005) 25(17): 4406-4419 
Categories:
Transfection 
Authors:
Meijer OC, Kalkhoven E, van der Laan S, Steenbergen PJ, Houtman SH, Dijkmans TF, Pearce D and de Kloet ER 
In:
Endocrinology (2005) 146(3): 1438-1448 
Categories:
Transfection 
Authors:
Impey S, McCorkle SR, Cha-Molstad H, Dwyer JM, Yochum GS, Boss JM, McWeeney S, Dunn JJ, Mandel G and Goodman RH 
In:
Cell (2004) 119(7): 1041-1054 
Categories:
Transfection 
Authors:
Bajohrs M, Rickman C, Binz T and Davletov B 
In:
EMBO Rep (2004) 5(11): 1090-1095 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
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