NIH/3T3

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Swiss NIH embryonic fibroblast

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R A-024 1e6 68% ±17 89% Plasmid (general) 1 µg 100 µl I/II/2b
SG EN-158 2e5 90% ±2 51% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SG EN-158 1e6 88.1% ±2 53% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
SG 96-EN-158 2e5 95% ±2 93% ±4 Plasmid (general) 0.4 µg 20 µl 96-well
R U-030 1e6 84% ±4 87% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (39)

Electroporation-mediated Delivery of Cas9Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes
Categories:
Transfection 
Authors:
Tanner Rathbone , Ilayda Ates , Lawrence Fernando , Ethan Addlestone , Ciaran M Lee , Vincent P Richards , Renee N Cottle de  
In:
CRISPR J (2022) :  
A 3D Heterotypic Multicellular Tumor Spheroid Assay Platform to Discriminate Drug Effects on Stroma versus Cancer Cells.
Categories:
3D Cell Culture , Transfection 
Authors:
Weydert Z, Lal-Nag M, Mathews-Greiner L, Thiel C, Cordes H, Küpfer L, Guye P, Kelm JM, Ferrer M. 
In:
SLAS Discov (2019) 1: 12 
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
Targeted genome modification in mice using zinc-finger nucleases. Genetics
Categories:
Transfection 
Authors:
Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui X 
In:
Genome Res (2010) 186(2): 451-9 
A pathway for phagosome maturation during engulfment of apoptotic cells
Categories:
Transfection 
Authors:
Kinchen JM, Doukoumetzidis K, Almendinger J, Stergiou L, Tosello-Trampont A, Sifri CD, Hengartner MO, Ravichandran KS 
In:
Nat Cell Biol (2008) 10(5): 556-66 
Heritable gene repression through the action of a directed DNA methyltransferase at a chromosomal locus
Categories:
Transfection 
Authors:
Smith AE, Hurd PJ, Bannister AJ, Kouzarides T, Ford KG 
In:
J Biol Chem (2008) 283(15): 9878-85 
GADD45A Does Not Promote DNA Demethylation
Categories:
Transfection 
Authors:
Jin SG, Guo C, Pfeifer GP 
In:
PLoS Genet (2008) 4(3): e1000013 
Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function
Categories:
Transfection 
Authors:
Vethantham V, Rao N, Manley JL 
In:
Genes Dev (2008) 22(4): 499-511 
The nuclear RhoA-exchange factor Net1 interacts with proteins of the Dlg family, affects their localization and influences their tumor suppressor activity
Categories:
Transfection 
Authors:
Garcia-Mata R, Dubash AD, Sharek L, Carr HS, Frost JA, Burridge K 
In:
Mol Cell Biol (2007) 27(24): 8683-97 
Retrograde Fluxes of Focal Adhesion Proteins in Response to Cell Migration and Mechanical Signals
Categories:
Transfection 
Authors:
Guo WH, Wang YL 
In:
Mol Biol Cell (2007) 18(11): 4519-4527 
Cyclosporin A inhibits mouse cytomegalovirus infection via a cyclophilin-dependent pathway specifically in neural stem/progenitor cells
Categories:
Transfection 
Authors:
Kawasaki H, Mocarski ES, Kosugi I, Tsutsui Y 
In:
J Virol (2007) 81(17): 9013-23 
The role of the poly(ADP-ribose) polymerase tankyrase1 in telomere length control by the TRF1 component of the shelterin complex
Categories:
Transfection 
Authors:
Donigian JR, de Lange T 
In:
J Biol Chem (2007) 282(31): 22662-7 
Identification of two signaling submodules within the CrkII/ELMO/Dock180 pathway regulating engulfment of apoptotic cells
Categories:
Transfection 
Authors:
Tosello-Trampont AC, Kinchen JM, Brugnera E, Haney LB, Hengartner MO, Ravichandran KS 
In:
Cell Death Differ (2007) 14(5): 963-72 
Polymeric meshes induce zonal regulation of matrix metalloproteinase-2 gene expression by macrophages and fibroblasts
Categories:
Transfection 
Authors:
Jansen PL, Kever M, Rosch R, Krott E, Jansen M, Alfonso-Jaume A, Dooley S, Klinge U, Lovett DH, Mertens PR 
In:
FASEB J (2007) 21(6): 1047-57 
Nuclear localization of the parafibromin tumor suppressor protein implicated in the hyperparathyroidism-jaw tumor syndrome enhances its proapoptotic function
Categories:
Transfection 
Authors:
Lin L, Czapiga M, Nini L, Zhang JH, Simonds WF 
In:
Mol Cancer Res (2007) 5(2): 183-93 
The Rab4a effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts
Categories:
Transfection 
Authors:
Vukmirica J, Monzo P, Le Marchand-Brustel Y, Cormont M 
In:
J Biol Chem (2006) 281(47): 36360-8 
A common signaling cascade may underlie "addiction" to the Src, BCR-ABL, and EGF receptor oncogenes
Categories:
Transfection 
Authors:
Sharma SV, Gajowniczek P, Way IP, Lee DY, Jiang J, Yuza Y, Classon M, Haber DA, Settleman J 
In:
Cancer Cell (2006) 10(5): 425-35 
Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways
Categories:
Transfection 
Authors:
Shibue T, Suzuki S, Okamoto H, Yoshida H, Ohba Y, Takaoka A, Taniguchi T 
In:
EMBO J (2006) 25(20): 4952-62 
Specific recognition of apoptotic cells reveals a ubiquitous and unconventional innate immunity
Categories:
Transfection 
Authors:
Cvetanovic M, Mitchell JE, Patel V, Avner BS, Su Y, van der Saag PT, Witte PL, Fiore S, Levine JS, Ucker DS 
In:
J Biol Chem (2006) 281(29): 20055-67 
SHP1 tyrosine phosphatase negatively regulates NPM-ALK tyrosine kinase signaling
Categories:
Transfection 
Authors:
Honorat JF, Ragab A, Lamant L, Delsol G, Ragab-Thomas J 
In:
Blood (2006) 107(10): 4130-8 
Cellular Responses to Substrate Topography: Role of Myosin II and Focal Adhesion Kinase
Categories:
Transfection 
Authors:
Frey MT, Tsai IY, Russell TP, Hanks SK, Wang YL 
In:
Biophys J (2006) 90(10): 3774-82 
The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells
Categories:
Transfection 
Authors:
Mitchell JE, Cvetanovic M, Tibrewal N, Patel V, Colamonici OR, Li MO, Flavell RA, Levine JS, Birge RB and Ucker DS 
In:
J Biol Chem (2006) 281(9): 5718-25 
Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress
Categories:
Transfection 
Authors:
Speidel D, Helmbold H and Deppert W 
In:
Oncogene (2006) 25(6): 940-53 
Matrix Contraction by Dermal Fibroblasts Requires Transforming Growth Factor-/Activin-Linked Kinase 5, Heparan Sulfate-Containing Proteoglycans, and MEK/ERK: Insights into Pathological Scarring in Chronic Fibrotic Disease
Categories:
Transfection 
Authors:
Chen Y, Shi-Wen X, van Beek J, Kennedy L, McLeod M, Renzoni EA, Bou-Gharios G, Wilcox-Adelman S, Goetinck PF, Eastwood M, Black CM, Abraham DJ and Leask A 
In:
Am J Pathol (2005) 167(6): 1699-1711 
Ceramide mediates caspase-independent programmed cell death
Categories:
Transfection 
Authors:
Thon L, Mohlig H, Mathieu S, Lange A, Bulanova E, Winoto-Morbach S, Schutze S, Bulfone-Paus S and Adam D 
In:
FASEB J (2005) 19(14): 1945-1956 
Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication
Categories:
Transfection 
Authors:
Wang W, Budhu A, Forgues M und Wang XW 
In:
Nat Cell Biol (2005) 7(8): 823-830 
Connexin43 associated with an N-cadherin containing multi-protein complex is required for gap junction formation in NIH3T3 cells
Categories:
Transfection 
Authors:
Wei CJ, Francis R, Xu X and Lo CW 
In:
J Biol Chem (2005) 280(20): 19925-19936 
Interaction of paxillin with poly(a)-binding protein 1 and its role in focal adhesion turnover and cell migration
Categories:
Transfection 
Authors:
Woods AJ, Kantidakis T, Sabe H, Critchley DR and Norman JC 
In:
Mol Cell Biol (2005) 25(9): 3763-3773 
Tumor necrosis factor-alpha induces transforming growth factor-beta1 expression in lung fibroblasts through the extracellular signal-regulated kinase pathway
Categories:
Transfection 
Authors:
Sullivan DE, Ferris M, Pociask D, Brody AR 
In:
Am J Respir Cell Mol Biol (2005) 32(4): 342-349 
TATA-binding protein (TBP)-like factor (TLF) is a functional regulator of transcription: reciprocal regulation of the neurofibromatosis type 1 and c-fos genes by TLF/TRF2 and TBP
Categories:
Transfection 
Authors:
Chong JA, Moran MM, Teichmann M, Kaczmarek JS, Roeder R and Clapham DE 
In:
Mol Cell Biol (2005) 25(7): 2632-2643 
Cyclin A1, the alternative A-type cyclin, contributes to G1/S cell cycle progression in somatic cells
Categories:
Transfection 
Authors:
Ji P, Agrawal S, Diederichs S, Baumer N, Becker A, Cauvet T, Kowski S, Beger C, Welte K, Berdel WE, Serve H and Muller-Tidow C 
In:
Oncogene (2005) 24(16): 2739-2744 
Apoptosis of the dermal papilla cells of hair follicle associated with the expression of gene HSPCO16 in vitro
Categories:
Transfection 
Authors:
Yang WB, Hao F, Song ZQ, Yang XC, Ni B 
In:
Exp Dermatol (2005) 14(3): 209-214 
Parafibromin, product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2, regulates cyclin D1/PRAD1 expression
Categories:
Transfection 
Authors:
Woodard GE, Lin L, Zhang JH, Agarwal SK, Marx SJ and Simonds WF 
In:
Oncogene (2005) 24(7): 1272-1276 
Ciz1 promotes mammalian DNA replication
Categories:
Transfection 
Authors:
Coverley D, Marr J and Ainscough J 
In:
J Cell Sci (2005) 118(Pt 1): 101-112 
Identification and modulation of a caveolae-dependent signal pathway that regulates plasminogen activator inhibitor-1 in insulin-resistant adipocytes
Categories:
Transfection 
Authors:
Venugopal J, Hanashiro K, Yang ZZ and Nagamine Y 
In:
Proc Natl Acad Sci USA (2004) 101(49): 17120-17125 
Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors
Categories:
Transfection 
Authors:
Beningo KA, Dembo M and Wang YL 
In:
Proc Natl Acad Sci USA (2004) 101(52): 18024-18029 
Molecular interactions of B-CAM (basal-cell adhesion molecule) and laminin in epithelial skin cancer
Categories:
Transfection 
Authors:
Drewniok C, Wienrich BG, Schon M, Ulrich J, Zen Q, Telen MJ, Hartig RJ, Wieland I, Gollnick H and Schon MP 
In:
Arch Dermatol Res (2004) 296(2): 59-66 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins
Categories:
Transfection 
Authors:
Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P and Norman JC 
In:
Mol Cell Biol (2004) 24(4): 1505-1515 
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