SMC, vascular, rat

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Smooth muscle cells isolated from rat vasculature
Cell Type:
Smooth muscle cell
Tissue Origin:
Species:
rat
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic U-025 1e6 76% 97% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (8)

Changes in the function of angiotensin II type 1 receptor due to cholesterol depletion from cell membrane
Categories:
Primary Cells and Media 
Authors:
Matsuo Y, Suematsu Y, Idemoto Y, Kuwano T, Kitajima K, Miura SI 
In:
Biochem Biophys Res Commun (2019) 514: 3 
Requirement of an intermediate gene expression for biphasic ERK1/2 activation in thrombin-stimulated vascular smooth muscle cells
Categories:
Transfection 
Authors:
Perez Sastre A, Grossmann S, Reusch HP, Schaefer M 
In:
J Biol Chem (2008) epub: online 
The transactivated EGF receptor recruits Pyk2 to regulate Src kinase activity
Categories:
Transfection 
Authors:
Schauwienold D, Pérez Sastre A, Genzel N, Schaefer M, Reusch HP 
In:
J Biol Chem (2008) epub: online 
Mitofusin 2 triggers vascular smooth muscle cell apoptosis via mitochondrial death pathway
Categories:
Transfection 
Authors:
Guo X, Chen KH, Guo Y, Liao H, Tang J, Xiao RP 
In:
Circ Res (2007) 101(11): 1113-22 
Notch3 and IL-1beta exert opposing effects on a vascular smooth muscle cell inflammatory pathway in which NF-kappaB drives crosstalk
Categories:
Transfection 
Authors:
Clement N, Gueguen M, Glorian M, Blaise R, Andreani M, Brou C, Bausero P, Limon I 
In:
J Cell Sci (2007) 120(Pt 19): 3352-61 
Apolipoprotein E Interrupts Interleukin-1 Signaling in Vascular Smooth Muscle Cells
Categories:
Transfection 
Authors:
Kawamura A, Baitsch D, Telgmann R, Feuerborn R, Weissen-Plenz G, Hagedorn C, Saku K, Brand-Herrmann SM, von Eckardstein A, Assmann G, Nofer JR 
In:
Arterioscler Thromb Vasc Biol (2007) 27(7): 1610-7 
Cooperation of SRC-1 and p300 with NF-kappaB and CREB in angiotensin II-induced IL-6 expression in vascular smooth muscle cells
Categories:
Transfection 
Authors:
Sahar S, Reddy MA, Wong C, Meng L, Wang M, Natarajan R 
In:
Arterioscler Thromb Vasc Biol (2007) 27(7): 1528-34 
AKT Phosphorylation Is Essential For Insulin-induced Relaxation of Rat Vascular Smooth Muscle Cells
Categories:
Transfection 
Authors:
Lee JH, Ragolia L 
In:
Am J Physiol Cell Physiol (2006) 291(6): C1355-65 
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