cells

human prostate carcinoma

Cell Type:

Prostate Epith.

Tissue Origin:

prostate

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	EN-120	1e6	70% ±2	43% ±1	Plasmid (general)	2 µg	100 µl	4D X-Unit
SF	EN-120	2e5	70% ±2	45% ±1	Plasmid (general)	400 ng	20 µl	4D X-Unit
R	T-009	2e6	70% ±15	70%	Plasmid (general)	2 µg	100 µl	I/II/2b
R	T-009	2e6	82% ±10	80%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (19)

CAUSEL: an epigenome- and genome-editing pipeline for establishing function of noncoding GWAS variants

Categories:

Transfection

Authors:

Sándor Spisák, Kate Lawrenson, Yanfang Fu, István Csabai, Rebecca T Cottman, Ji-Heui Seo, Christopher Haiman, Ying Han, Romina Lenci, Qiyuan Li, Viktória Tisza, Zoltán Szállási, Zachery T Herbert, Matthew Chabot, Mark Pomerantz, Norbert Solymosi, The GAME-ON/ELLIPSE Consortium, Simon A Gayther, J Keith Joung, Matthew L Freedman

In:

Nat Med (2015) 21(11): 1357-63

Steroidal Androgens and Nonsteroidal, Tissue-Selective Androgen Receptor Modulator, S-22, Regulate Androgen Receptor Function through Distinct Genomic and Nongenomic Signaling Pathways

Categories:

Transfection

Authors:

Narayanan R, Coss CC, Yepuru M, Kearbey JD, Miller DD, Dalton JT

In:

Mol Endocrinol (2008) 22(11): 2448-65

Target Gene-Specific Regulation of Androgen Receptor Activity by p42/p44 Mitogen-Activated Protein Kinase

Categories:

Transfection

Authors:

Agoulnik IU, Bingman WE 3rd, Nakka M, Li W, Wang Q, Liu XS, Brown M, Weigel NL

In:

Mol Endocrinol (2008) 22(11): 2420-32

Multiple Cellular Mechanisms Related to Cyclin A1 in Prostate Cancer Invasion and Metastasis

Categories:

Transfection

Authors:

Wegiel B, Bjartell A, Tuomela J, Dizeyi N, Tinzl M, Helczynski L, Nilsson E, Otterbein LE, Harkonen P, Persson JL

In:

J Natl Cancer Inst (2008) 100(14): 1022-36

HDAC6 is a specific deacetylase of peroxiredoxins and is involved in redox regulation

Categories:

Transfection

Authors:

Parmigiani RB, Xu WS, Venta-Perez G, Erdjument-Bromage H, Yaneva M, Tempst P, Marks PA

In:

Proc Natl Acad Sci USA (2008) 105(28): 9633-8

A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells

Categories:

Transfection

Authors:

Wei S, Yang HC, Chuang HC, Yang J, Kulp SK, Lu PJ, Lai MD, Chen CS

In:

J Biol Chem (2008) epub: online

Expression of androgen receptor is negatively regulated by p53

Categories:

Transfection

Authors:

Alimirah F, Panchanathan R, Chen J, Zhang X, Ho SM, Choubey D

In:

Neoplasia (2007) 9(12): 1152-9

The Zinc Finger Protein RREB-1 is a Co-Regulator of the Androgen Receptor: Implications for the Role of the Ras Pathway in Enhancing Androgenic Signaling in Prostate Cancer

Categories:

Transfection

Authors:

Mukhopadhyay NK, Cinar B, Mukhopadhyay L, Lutchman M, Ferdinand AS, Kim J, Chung LW, Adam RM, Ray SK, Leiter AB, Richie JP, Liu BC, Freeman MR

In:

Mol Endocrinol (2007) 21(9): 2056-2070

Vasoactive intestinal peptide transactivates the androgen receptor through a PKA-dependent extracellular signal-regulated kinase pathway in prostate cancer LNCaP cells

Categories:

Transfection

Authors:

Xie Y, Wolff DW, Lin MF, Tu Y

In:

Mol Pharmacol (2007) 72(1): 73-85

Activated Cdc42-associated kinase Ack1 promotes prostate cancer progression via androgen receptor tyrosine phosphorylation

Categories:

Transfection

Authors:

Mahajan NP, Liu Y, Majumder S, Warren MR, Parker CE, Mohler JL, Earp HS, Whang YE

In:

Proc Natl Acad Sci USA (2007) 104(20): 8438-43

Peroxisome Proliferator-Activated Receptor -Independent Suppression of Androgen Receptor Expression by Troglitazone Mechanism and Pharmacologic Exploitation

Categories:

Transfection

Authors:

Yang CC, Wang YC, Wei S, Lin LF, Chen CS, Lee CC, Lin CC, Chen CS

In:

Cancer Res (2007) 67(7): 3229-38

IFI16 in Human Prostate Cancer

Categories:

Transfection

Authors:

Alimirah F, Chen J, Davis FJ, Choubey D

In:

Mol Cancer Res (2007) 5(3): 251-9

Relaxin promotes prostate cancer progression

Categories:

Transfection

Authors:

Feng S, Agoulnik IU, Bogatcheva NV, Kamat AA, Kwabi-Addo B, Li R, Ayala G, Ittmann MM, Agoulnik AI

In:

Clin Cancer Res (2007) 13(6): 1695-702

Cannabinoid receptor agonist induced apoptosis of human prostate cancer cells LNCaP proceeds through sustained activation of ERK1/2 leading to G1 cell cycle arrest

Categories:

Transfection

Authors:

Sarfaraz S, Afaq F, Adhami VM, Malik A, Mukhtar H

In:

J Biol Chem (2006) 281(51): 39480-91

Androgens Modulate Expression of Transcription Intermediary Factor 2, an Androgen Receptor Coactivator whose Expression Level Correlates with Early Biochemical Recurrence in Prostate Cancer

Categories:

Transfection

Authors:

Agoulnik IU, Vaid A, Nakka M, Alvarado M, Bingman WE 3rd, Erdem H, Frolov A, Smith CL, Ayala GE, Ittmann MM, Weigel NL

In:

Cancer Res (2006) 66(21): 10594-602

Regulation of apoptosis by a prostate-specific and prostate cancer-associated noncoding gene, PCGEM1

Categories:

Transfection

Authors:

Fu X, Ravindranath L, Tran N, Petrovics G, Srivastava S

In:

DNA Cell Biol (2006) 25(3): 135-41

The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase

Categories:

Transfection

Authors:

Johnson JL, Ellis BA, Noack D, Seabra MC, Catz SD

In:

Biochem J (2005) 391(Pt 3): 699-710

A role for cyclin A1 in mediating the autocrine expression of vascular endothelial growth factor in prostate cancer

Categories:

Transfection

Authors:

Wegiel B, Bjartell A, Ekberg J, Gadaleanu V, Brunhoff C and Persson JL

In:

Oncogene (2005) 24(42): 6385-6393

The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines

Categories:

Live Cell Imaging

Authors:

Orellana-Serradell O1, Herrera D1, Castellon EA1, Contreras HR1.

In:

Asian J Androl () 3: 294-299

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