RIN m5f

islet of Langerhans cells

Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DE-130 1e6 68% ±1 72% ±5 Plasmid (general) 1.2 µg 100 µl 4D X-Unit
SF DE-130 2e5 90% 85% ±3 Plasmid (general) 600 ng 20 µl 4D X-Unit
SF DE-130 2e5 90% 85% ±3 Plasmid (general) 600 ng 20 µl Shuttle

Citations

Categories:
Transfection 
Authors:
Ning Y, O'Neill K, Lan H, Pang L, Shan LX, Hawes BE, Hedrick JA 
In:
Br J Pharmacol (2008) epub: online 
Categories:
Transfection 
Authors:
Chambers KT, Weber SM, Corbett JA 
In:
Am J Physiol Endocrinol Metab (2007) 292(4): E1052-61