cells

H9c2(2-1) myoblast cell line is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.

Cell Type:

Myoblast

Tissue Origin:

heart

Species:

rat

Research Area:

Cardiovascular

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	DS-120	1e6	90%	54%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SF	DS-120	2e5	80%	72%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
L	C-020	4e5	86% ±4	90% ±4	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Mutations in filamin C cause a new form of familial hypertrophic cardiomyopathy.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Valdés-Mas R, Gutiérrez-Fernández A, Gómez J, Coto E, Astudillo A, Puente DA, Reguero JR, Alvarez V, Morís C, León D, Martín M, Puente XS, López-Otín C.

In:

Nat Commun. (2014) 5: 5326

Metformin protects cardiomyocyte from doxorubicin induced cytotoxicity through an AMP-activated protein kinase dependent signaling pathway: an in vitro study.

Categories:

Authors:

Kobashigawa LC, Xu YC, Padbury JF, Tseng YT, Yano N.

In:

PLoS ONE (2014) 9(8): ePub