K-562

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human CML-derived B cell like

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-016 1e6 89% ±3 88% Plasmid (general) 1 µg 100 µl I/II/2b
V T-003 1e6 79% ±5 89% Plasmid (general) 1 µg 100 µl I/II/2b
SF 96-FF-120 2e5 92% ±3 95% ±2 Plasmid (general) 0.4 µg 20 µl 96-well
SF

FF-120

 2e5 89-95% 93-97% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

FF-120

 1e6 89-95% 93-97% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (86)

Gene editing of hematopoietic stem cells restores T-cell response in familial hemophagocytic lymphohistiocytosis
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Dettmer-Monaco V, Weißert K, Ammann S, Monaco G, Lei L, Gräßel L, Rhiel M, Rositzka J, Kaufmann MM, Geiger K, Andrieux G, Lao J, Thoulass G, Schell C, Boerries M, Illert AL, Cornu TI, Ehl S, Aichele P, Cathomen T 
In:
J Allergy Clin Immunol (2023) S0091: 6749(23)00989-2 
A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Carusillo A, Haider S, Schäfer R, Rhiel M, Türk D, Chmielewski KO, Klermund J, Mosti L, Andrieux G, Schäfer R, Cornu TI, Cathomen T, Mussolino C 
In:
Nucleic Acids Res (2023) 51(9): 4660-4673 
CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA 
In:
Nucleic Acids Res (2022) 50(3): 1256-1268 
Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning
Categories:
Transfection 
Authors:
Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR. 
In:
Nat Biotechnol (2021) 39(11): 1414-1425 
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
Categories:
Transfection 
Authors:
Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grünewald J, Joung JK 
In:
Nat Biotechnol (2021) Jan;39(1): 41-46 
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
Categories:
Transfection 
Authors:
Kluesner MG, Lahr WS, Lonetree CL, Smeester BA, Qiu X, Slipek NJ, Claudio Vázquez PN, Pitzen SP, Pomeroy EJ, Vignes MJ, Lee SC, Bingea SP, Andrew AA, Webber BR, Moriarity BS 
In:
Nature (2021) 12(1): 2437 
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
Categories:
Transfection 
Authors:
Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR. 
In:
Scientific Reports (2021) 11(1): 19482 
CRISPR off-target detection with DISCOVER-seq
Categories:
Transfection 
Authors:
Wienert B, Wyman SK, Yeh CD, Conklin BR, Corn JE 
In:
Nat Protocols (2020) 15(5): 1775-1799 
Search-and-replace genome editing without double-strand breaks or donor DNA.
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Anzalone AV1,2,3, Randolph PB1,2,3, Davis JR1,2,3, Sousa AA1,2,3, Koblan LW1,2,3, Levy JM1,2,3, Chen PJ1,2,3, Wilson C1,2,3, Newby GA1,2,3, Raguram A1,2,3, Liu DR4,5,6. 
In:
Nature (2019) 10: 1038 
Highly efficient editing of the ß-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease
Categories:
Transfection 
Authors:
So Hyun Park , Ciaran M Lee , Daniel P Dever , Timothy H Davis , Joab Camarena , Waracharee Srifa , Yankai Zhang , Alireza Paikari , Alicia K Chang , Matthew H Porteus , Vivien A Sheehan , Gang Bao  
In:
Nucleic Acids Res (2019) 47(15): 7955-7972 
Bacteria-free minicircle DNA system to generate integration-free CAR-T cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.
Categories:
Transfection 
Authors:
Bak RO, Porteus MH. 
In:
Cell Rep (2017) 20(3): 750-756 
Marker-free coselection for CRISPR-driven genome editing in human cells.
Categories:
Transfection 
Authors:
Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganière J, Doyon Y. 
In:
Nat Methods (2017) 14(6): 615-620 
MiRNA182 regulates percentage of myeloid and erythroid cells in chronic myeloid leukemia
Categories:
Transfection 
Authors:
Arya D, Sachithanandan SP, Ross C, Palakodeti D, Li S, Krishna S. 
In:
Cell Death Dis. (2017) 2017;8(1): e2547 
Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.
Categories:
Transfection 
Authors:
DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo SJ, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DI, Corn JE. 
In:
Science (2016) 8 (360): 1-9 
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
TALENs-mediated gene disruption of FLT3 in leukemia cells: Using genome-editing approach for exploring the molecular basis of gene abnormality.
Categories:
Transfection 
Authors:
Wang J, Li T, Zhou M, Hu Z, Zhou X, Zhou S, Wang N, Huang L, Zhao L, Cao Y, Xiao M, Ma D, Zhou P, Shang Z, Zhou J 
In:
Scientific Reports (2015) 5:18454: 10.1038 
A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
Categories:
 
Authors:
Mathieu Dalvai, Jeremy Loehr, Karine Jacquet, Caroline C. Huard, Céline Roques, Pauline Herst, Jacques Côté , Yannick Doyon 
In:
Cell Rep (2015) 13(3): 621-33 
Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Breese EH, Buechele C, Dawson C, Cleary ML, Porteus MH. 
In:
PLoS ONE (2015) 10(9: e0136644 
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
Categories:
Transfection 
Authors:
Raj Chari, Prashant Mali, Mark Moosburner, George M Church 
In:
Nat Methods (2015) 12(9): 823-6 
Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9
Categories:
Transfection 
Authors:
Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS, Stortchevoi A, Bryder D, Musunuru K, Brand H, Tager AM, Allen TM, Talkowski ME, Rossi DJ, Cowan CA 
In:
Cell Stem Cell (2014) 15(5): 643-652 
Hydroxyurea-inducible SAR1 gene acts through the Gia/JNK/Jun pathway to regulate ?-globin expression.
Categories:
Primary Cells and Media, Transfection 
Authors:
Zhu J, Chin K, Aerbajinai W, Kumkhaek C, Li H, Rodgers GP 
In:
Blood (2014) 124(7): 1146-56 
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
Categories:
Transfection 
Authors:
Kim S, Kim D, Cho SW, Kim J, Kim JS 
In:
Genome Res (2014) 24(6): 1012-9 
High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.
Categories:
Transfection 
Authors:
Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M. 
In:
Nucleic Acids Res (2014) 42: 1-16 
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
Categories:
Transfection 
Authors:
Cho SW, Kim S, Kim Y, Kweon J, Kim HS, Bae S, Kim JS 
In:
Genome Res (2014) 24(1): 132–141 
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
Categories:
Transfection 
Authors:
Yanfang Fu, Jennifer A Foden, Cyd Khayter, Morgan L Maeder, Deepak Reyon, J Keith Joung, Jeffry D Sander 
In:
Nat Biotechnol (2013) 31(9): 822-6 
RNA-guided human genome engineering via Cas9
Categories:
Transfection 
Authors:
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM 
In:
Science (2013) 339(6121): 823-826 
Precision genome engineering with programmable DNA-nicking enzymes
Categories:
Transfection 
Authors:
Kim E, Kim S, Kim DH, Choi BS, Choi IY, Kim JS 
In:
Genome Res (2012) 22(7): 1327(6) 
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme
Categories:
 
Authors:
Jianbin Wang, Geoffrey Friedman, Yannick Doyon, Nathaniel S. Wang, Carrie Jiaxin Li, Jeffrey C. Miller, Kevin L. Hua, Jenny Jiacheng Yan, Joshua E. Babiarz, Philip D. Gregory, and Michael C. Holmes 
In:
Genome Res (2012) 22 (7): 1316-26 
The forkhead transcription factor FOXO3a increases phosphoinositide-3 kinase/Akt activity in drug-resistant leukemic cells through induction of PIK3CA expression
Categories:
Transfection 
Authors:
Hui RC, Gomes AR, Constantinidou D, Costa JR, Karadedou CT, Fernandez de Mattos S, Wymann MP, Brosens JJ, Schulze A, Lam EW 
In:
Mol Cell Biol (2008) 28(19): 5886-98 
Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells
Categories:
Transfection 
Authors:
Azad AK, Torrelles JB, Schlesinger LS 
In:
J Leukoc Biol (2008) epub: online 
RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma
Categories:
Transfection 
Authors:
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH 
In:
Gene Ther (2008) 15(13): 955-65 
Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels
Categories:
Transfection 
Authors:
Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N 
In:
Cancer Res (2008) 68(13): 5076-85 
upar induces conformational changes in the integrin alphambeta2 headpiece and re-orientation of its transmembrane domains
Categories:
Transfection 
Authors:
Tang ML, Vararattanavech A, Tan SM 
In:
J Biol Chem (2008) epub: online 
Human AQP5 plays a role in the progression of chronic myelogenous leukemia (CML)
Categories:
Transfection 
Authors:
Chae YK, Kang SK, Kim MS, Woo J, Lee J, Chang S, Kim DW, Kim M, Park S, Kim I, Keam B, Rhee J, Koo NH, Park G, Kim SH, Jang SE, Kweon IY, Sidransky D, Moon C 
In:
PLoS ONE (2008) 3(7): e2594 
Expression of myeloperoxidase enhances the chemosensitivity of leukemia cells through the generation of reactive oxygen species and the nitration of protein
Categories:
Transfection 
Authors:
Sawayama Y, Miyazaki Y, Ando K, Horio K, Tsutsumi C, Imanishi D, Tsushima H, Imaizumi Y, Hata T, Fukushima T, Yoshida S, Onimaru Y, Iwanaga M, Taguchi J, Kuriyama K, Tomonaga M 
In:
Leukemia (2008) 22(5): 956-64 
Subversion of CtBP1-controlled macropinocytosis by human adenovirus serotype 3
Categories:
Transfection 
Authors:
Amstutz B, Gastaldelli M, Kälin S, Imelli N, Boucke K, Wandeler E, Mercer J, Hemmi S, Greber UF 
In:
EMBO J (2008) 27(7): 956-69 
Regulating gene expression in human leukemia cells using light-activated oligodeoxynucleotides
Categories:
Transfection 
Authors:
Tang X, Swaminathan J, Gewirtz AM, Dmochowski IJ 
In:
Nucleic Acids Res (2008) 36(2): 559-69 
Expression of the CTCF-paralogous cancer-testis gene, brother of the regulator of imprinted sites (BORIS), is regulated by three alternative promoters modulated by CpG methylation and by CTCF and p53 transcription factors
Categories:
Transfection 
Authors:
Renaud S, Pugacheva EM, Delgado MD, Braunschweig R, Abdullaev Z, Loukinov D, Benhattar J, Lobanenkov V 
In:
Nucleic Acids Res (2007) 35(21): 7372-88 
Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9
Categories:
Transfection 
Authors:
Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G 
In:
Blood (2007) 110(9): 3301-9 
Novel RUNX1 isoforms determine the fate of acute myeloid leukemia cells by controlling CD56 expression
Categories:
Transfection 
Authors:
Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A 
In:
Blood (2007) 110(6): 2027-33 
A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) through 15-deoxy-12, 14 prostaglandin J2 production
Categories:
Transfection 
Authors:
Hasegawa H, Yamada Y, Komiyama K, Hayashi M, Ishibashi M, Sunazuka T, Izuhara T, Sugahara K, Tsuruda K, Masuda M, Takasu N, Tsukasaki K, Tomonaga M, Kamihira S 
In:
Blood (2007) 110(5): 1664-74 
Hip is a pro-survival substrate of granzyme B
Categories:
Transfection 
Authors:
Hostetter DR, Loeb CR, Chu F, Craik CS 
In:
J Biol Chem (2007) 282(38): 27865-74 
Mammalian NOTCH-1 activates beta1 integrins via the small GTPase R-Ras
Categories:
Transfection 
Authors:
Hodkinson PS, Elliot PA, Lad Y, McHugh BJ, Mackinnon AC, Haslett C, Sethi T 
In:
J Biol Chem (2007) 282(39): 28991-9001 
Myeloperoxidase Is a Key Regulator of Oxidative Stress Mediated Apoptosis in Myeloid Leukemic Cells
Categories:
Transfection 
Authors:
Nakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M 
In:
Clin Cancer Res (2007) 13(18): 5436-5445 
Cytokine-Independent Upregulation of MDA5 in Viral Infection
Categories:
Transfection 
Authors:
Yount JS, Moran TM, Lopez CB 
In:
J Virol (2007) 81(13): 7316-9 
An improved zinc-finger nuclease architecture for highly specific genome editing
Categories:
Transfection 
Authors:
Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ 
In:
Nat Biotechnol (2007) 25(7): 778-85 
Synergistic interactions between vorinostat and sorafenib in chronic myelogenous leukemia cells involve Mcl-1 and p21CIP1 down-regulation
Categories:
Transfection 
Authors:
Dasmahapatra G, Yerram N, Dai Y, Dent P, Grant S 
In:
Clin Cancer Res (2007) 13(14): 4280-90 
MicroRNA gene expression during retinoic acid-induced differentiation of human acute promyelocytic leukemia
Categories:
Transfection 
Authors:
Garzon R, Pichiorri F, Palumbo T, Visentini M, Aqeilan R, Cimmino A, Wang H, Sun H, Volinia S, Alder H, Calin GA, Liu CG, Andreeff M, Croce CM 
In:
Oncogene (2007) 26(28): 4148-57 
Proteomic Analysis Identifies Oxidative Stress Induction by Adaphostin
Categories:
Transfection 
Authors:
Stockwin LH, Bumke MA, Yu SX, Webb SP, Collins JR, Hollingshead MG, Newton DL 
In:
Clin Cancer Res (2007) 13(12): 3667-3681 
MEK1/2 inhibitors sensitize BCR/ABL+ human leukemia cells to the dual ABL/SRC inhibitor BMS354825
Categories:
Transfection 
Authors:
Nguyen TK, Rahmani M, Harada H, Dent P, Grant S 
In:
Blood (2007) 109(9): 4006-15 
The chromatin remodeling factor Mi-2alpha acts as a novel co-activator for human c-Myb
Categories:
Transfection 
Authors:
Saether T, Berge T, Ledsaak M, Matre V, Alm-Kristiansen AH, Dahle O, Aubry F, Gabrielsen OS 
In:
J Biol Chem (2007) 282(19): 13994-4005 
Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21(WAF1/CIP1).
Categories:
Primary Cells and Media 
Authors:
Alcantara O, Boldt DH 
In:
Br J of Haematol (2007) 137(3): 252-61 
Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity
Categories:
Transfection 
Authors:
Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL 
In:
Proc Natl Acad Sci USA (2007) 104(15): 6329-34 
c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46
Categories:
Transfection 
Authors:
Walzer T, Blery M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, Andre P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagne F, Vivier E 
In:
Proc Natl Acad Sci USA (2007) 104(9): 3384-9 
Overexpression of the heat-shock protein 70 is associated to imatinib resistance in chronic myeloid leukemia
Categories:
Transfection 
Authors:
Pocaly M, Lagarde V, Etienne G, Ribeil JA, Claverol S, Bonneu M, Moreau-Gaudry F, Guyonnet-Duperat V, Hermine O, Melo JV, Dupouy M, Turcq B, Mahon FX, Pasquet JM 
In:
Leukemia (2007) 21(1): 93-101 
Histone deacetylase inhibitors deplete enhancer of zeste 2 and associated polycomb repressive complex 2 proteins in human acute leukemia cells
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K 
In:
Mol Cancer Ther (2006) 5(12): 3096-104 
Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE
Categories:
Transfection 
Authors:
Chen L, Zhang H, Shi Y, Chin KL, Tang DC, Rodgers GP 
In:
Cell Res (2006) 16(12): 923-39 
A sequential blockade strategy for the design of combination therapies to overcome oncogene addiction in chronic myelogenous leukemia
Categories:
Transfection 
Authors:
Chen R, Gandhi V, Plunkett W 
In:
Cancer Res (2006) 66(22): 10959-10966 
Identification of 12/15-lipoxygenase as a suppressor of myeloproliferative disease
Categories:
Transfection 
Authors:
Middleton MK, Zukas AM, Rubinstein T, Jacob M, Zhu P, Zhao L, Blair I, Pure E 
In:
J Exp Med (2006) 203(11): 2529-40 
Gene therapy for BCR-ABL+ human CML with dual phosphorylation resistant p27Kip1 and stable RNA interference using an EBV vector
Categories:
Transfection 
Authors:
Sengupta A, Banerjee D, Chandra S, Banerjee S 
In:
J Gene Med (2006) 8(10): 1251-61 
Stable gene transfer to human CD34(+) hematopoietic cells using the Sleeping Beauty transposon
Categories:
Transfection 
Authors:
Hollis RP, Nightingale SJ, Wang X, Pepper KA, Yu XJ, Barsky L, Crooks GM, Kohn DB 
In:
Exp Hematol (2006) 34(10): 1333-43 
A novel mechanism for BCR-ABL action: stimulated secretion of CCN3 is involved in growth and differentiation regulation
Categories:
Transfection 
Authors:
McCallum L, Price S, Planque N, Perbal B, Pierce A, Whetton AD, Irvine AE 
In:
Blood (2006) 108(5): 1716-23 
Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells
Categories:
Transfection 
Authors:
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K 
In:
Leukemia (2006) 20(9): 1551-6 
Antagonistic Regulation of -Globin Gene Expression by Helix-Loop-Helix Proteins USF and TFII-I
Categories:
Transfection 
Authors:
Crusselle-Davis VJ, Vieira KF, Zhou Z, Anantharaman A, Bungert J 
In:
Mol Cell Biol (2006) 26(18): 6832-43 
Different roles for the TOS and RAIP motifs of the translational regulator protein 4E-BP1 in the association with raptor and phosphorylation by mTOR in the regulation of cell size
Categories:
Transfection 
Authors:
Eguchi S, Tokunaga C, Hidayat S, Oshiro N, Yoshino K, Kikkawa U, Yonezawa K 
In:
Genes Cells (2006) 11(7): 757-66 
Preclinical Assessment of FHIT Gene Replacement Therapy in Human Leukemia Using a Chimeric Adenovirus, Ad5/F35
Categories:
Transfection 
Authors:
Pichiorri F, Trapasso F, Palumbo T, Aqeilan RI, Drusco A, Blaser BW, Iliopoulos D, Caligiuri MA, Huebner K, Croce CM 
In:
Clin Cancer Res (2006) 12(11): 3494-501 
BCR/ABL Oncogene Directly Controls MHC Class I Chain-Related Molecule A Expression in Chronic Myelogenous Leukemia
Categories:
Transfection 
Authors:
Boissel N, Rea D, Tieng V, Dulphy N, Brun M, Cayuela JM, Rousselot P, Tamouza R, Le Bouteiller P, Mahon FX, Steinle A, Charron D, Dombret H, Toubert A 
In:
J Immunol (2006) 176(8): 5108-5116 
Synergistic Interactions between DMAG and Mitogen-Activated Protein Kinase Kinase 1/2 Inhibitors in Bcr/abl+ Leukemia Cells Sensitive and Resistant to Imatinib Mesylate
Categories:
Transfection 
Authors:
Nguyen TK, Rahmani M, Gao N, Kramer L, Corbin AS, Druker BJ, Dent P, Grant S 
In:
Clin Cancer Res (2006) 12(7): 2239-2247 
Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane
Categories:
Transfection 
Authors:
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ 
In:
J Cell Biol (2006) 172(6): 923-35 
MicroRNA fingerprints during human megakaryocytopoiesis
Categories:
Transfection 
Authors:
Garzon R, Pichiorri F, Palumbo T, Iuliano R, Cimmino A, Aqeilan R, Volinia S, Bhatt D, Alder H, Marcucci G, Calin GA, Liu CG, Bloomfield CD, Andreeff M, Croce CM 
In:
Proc Natl Acad Sci USA (2006) 103(13): 5078-83 
The hydroxyurea-induced small GTP-binding protein SAR modulates -globin gene expression in human erythroid cells
Categories:
Transfection 
Authors:
Tang DC, Zhu J, Liu W, Chin K, Sun J, Chen L, Hanover JA and Rodgers GP 
In:
Blood (2005) 94(5): 3278-3291 
Rationally targeted, conformationally constrained, oxetane-modified oligonucleotides demonstrate efficient gene-silencing activity in a cellular system
Categories:
Transfection 
Authors:
Opalinska JB, Gewirtz AM 
In:
Ann NY Acad Sci (2005) 1058: 39-51 
Abrogation of heat shock protein 70 induction as a strategy to increase antileukemia activity of heat shock protein 90 inhibitor 17-allylamino-demethoxy geldanamycin
Categories:
Transfection 
Authors:
Guo F, Rocha K, Bali P, Pranpat M, Fiskus W, Boyapalle S, Kumaraswamy S, Balasis M, Greedy B, Armitage ES, Lawrence N, Bhalla K 
In:
Cancer Res (2005) 65(22): 10536-44 
Apoptosis Induced by the Kinase Inhibitor BAY 43-9006 in Human Leukemia Cells Involves Down-regulation of Mcl-1 through Inhibition of Translation
Categories:
Transfection 
Authors:
Rahmani M, Maynard Davis E, Bauer C, Dent P and Grant S 
In:
J Biol Chem (2005) 280(42): 35217-35227 
Synergistic interactions between MEK1/2 and histone deacetylase inhibitors in BCR/ABL+ human leukemia cells
Categories:
Transfection 
Authors:
Yu C, Dasmahapatra G, Dent P and Grant S 
In:
Leukemia (2005) 19(9): 1579-1589 
Inhibition of histone deacetylase 6 acetylates and disrupts the chaperone function of heat shock protein 90: a novel basis for antileukemia activity of histone deacetylase inhibitors
Categories:
Transfection 
Authors:
Bali P, Pranpat M, Bradner J, Balasis M, Fiskus W, Guo F, Rocha K, Kumaraswamy S, Boyapalle S, Atadja P, Seto E and Bhalla K 
In:
J Biol Chem (2005) 280(29): 26729-26734 
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Categories:
Transfection 
Authors:
Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD and Holmes MC 
In:
Nature (2005) 435(7042): 646-651 
Inhibitory effect of c-Myc on p53-induced apoptosis in leukemia cells. Microarray analysis reveals defective induction of p53 target genes and upregulation of chaperone genes
Categories:
Transfection 
Authors:
Ceballos E, Munoz-Alonso MJ, Berwanger B, Acosta JC, Hernandez R, Krause M, Hartmann O, Eilers M and Leon J 
In:
Oncogene (2005) 24(28): 4559-4571 
Cotreatment with Suberanoylanilide Hydroxamic Acid and 17-Allylamino 17-demethoxygeldanamycin Synergistically Induces Apoptosis in Bcr-Abl+ Cells Sensitive and Resistant to STI571 (Imatinib Mesylate) in Association with Down-Regulation of Bcr-Abl, Abrogation of Signal Transducer and Activator of Transcription 5 Activity, and Bax Conformational Change
Categories:
Transfection 
Authors:
Rahmani M, Reese E, Dai Y, Bauer C, Kramer LB, Huang M, Jove R, Dent P and Grant S 
In:
Mol Pharmacol (2005) 67(4): 1166-1176 
SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc
Categories:
Transfection 
Authors:
Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M 
In:
FASEB J (2005) 272(7): 1590-1599 
Role of Chk1 and Chk2 in Ara-C-induced differentiation of human leukemia K562 cells
Categories:
Transfection 
Authors:
Takagaki K, Katsuma S, Kaminishi Y, Horio T, Tanaka T, Ohgi T and Yano J 
In:
Genes Cells (2005) 10(2): 97-106 
Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
Neuromedin U: a Myb-regulated autocrine growth factor for human myeloid leukemias
Categories:
Transfection 
Authors:
Shetzline SE, Rallapalli R, Dowd KJ, Zou S, Nakata Y, Swider CR, Kalota A, Choi JK and Gewirtz AM 
In:
Blood (2004) 104(6): 1833-1840 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
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