mIMCD3

kidney, medulla; collecting duct; SV40 transformed

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-020 1e6 68% 86% Plasmid (general) 5 µg 100 µl I/II/2b
R T-024 1e6 70% 87% Plasmid (general) 5 µg 100 µl I/II/2b
R U-017 1e6 70% 91% Plasmid (general) 5 µg 100 µl I/II/2b
R U-020 1e6 68% 88% Plasmid (general) 5 µg 100 µl I/II/2b
SF FP-100 2e5 90% 80% Plasmid (general) 400 ng 20 µl Shuttle
SF DS-137 2e5 90% 70% Plasmid (general) 400 ng 20 µl Shuttle
SF DS-130 2e5 90% 70% Plasmid (general) 400 ng 20 µl Shuttle

Citations

Categories:
Transfection 
Authors:
Goel M, Sinkins WG, Zuo CD, Hopfer U, Schilling WP 
In:
Am J Physiol Renal Physiol (2007) 293(5): F1476-88 
Categories:
Transfection 
Authors:
Mukhin YV, Gooz M, Raymond JR, Garnovskaya MN 
In:
J Pharmacol Exp Ther (2006) 318(3): 1033-43